A new cucurbit[6]uril-based ion-selective electrode for acetylcholine with high selectivity over choline and related quaternary ammonium ions

摘要

Detection of acetylcholine (ACh +) with high sensitivity and selectivity in the presence of interfering ions including choline (Ch +) poses a significant analytical challenge. A cucurbit[6]uril derivative, (allyloxy) 12CB[6], forms a stable 1:1 inclusion complex with ACh + but does not interact significantly with Ch + in solution. Based on this result, we prepared ion-selective electrodes (ISEs) for ACh + using (allyloxy) 12CB[6] as an ionophore. The (allyloxy) 12CB[6]-based ISEs detect ACh + with unprecedentedly high selectivity over Ch + as well as other interfering ions such as dopamine, NH 4 +, N(CH 3) 4 + and N(CH 2CH 3) 4 +.