Hexanal-induced Changes in miRNA-mRNA Interactions in A549 Human Alveolar Epithelial Cells

摘要

Although hexanal is considered to be a major air pollutant the correlation between hexanal and health risk is largely unknown. Identifying aldehyde toxicity in eukaryotic cells will be useful for preventing and treating environmental diseases and disorders. However, it is important to note that previous microRNA (miRNA) studies of hexanal have not yet identified the functional relationship between miRNA and mRNA. The aim of this study is to investigate the modulation of the integrated miRNA-mRNA relationship following exposure to hexanal. A549 human alveolar epithelial cells were treated to a 20% inhibitory concentration (IC20) of hexanal for 48 h. The microarray analysis results showed that 6 miRNA were altered in the hexanal-exposed A549 cells. Integrated analysis of miRNA and mRNA expression profiles identified 445 miRNA-mRNA correlations. KEGG analysis of 445 putative target genes of hexanal-induced miRNAs indicated that 8 genes (ATP2B3, ATP2B4, CACNA1G, EDNRA, GRM5, ITPR2, ITPKB, PTGER3) are involved in the calcium signaling pathway, which plays an important role in Alzheimer's and several inherited immunodeficiency diseases. Therefore, this study suggests the value of miRNA-mRNA networks in order to identify molecular regulation in responseto hexanal exposure in an in vitro model. Furthermore, this approach provides insight into the potential biological process of toxicity and a better understanding of the toxicol-ogical mechanism of toxicants.