COMPARATIVE INVESTIGATIONS ON THE EFFECT OF ENVIRONMENTAL SALT STRESS ON ENERGY METABOLISM OF AMPHIPODS ESTIMATED BY P-31 NMR SPECTROSCOPY AND MICROCALORIMETRY

摘要

Potassium pollution of rivers like the Werra, Weser and Rhine directly affects the energy metabolism of indigenous amphipods. After environmental pollution by KCI (10.0 mmol l(-1)), the heat dissipation of Gammarus pulex and G. tigrinus, estimated by microcalorimetry, increases significantly in two steps, Using P-31 NMR spectroscopy, the metabolic origin of the increasing heat production of G. pulex and G. tigrinus during KCl pollution of their incubation was investigated. Tn both species there is an initial increase in aerobic metabolism after the switch to 10.0 mmol l(-1) K+ stress. During prolonged K+ stress an additional increase in heat dissipation, correlated to a simultaneous decrease in phospho-L-arginine in both species, is caused by anaerobic metabolism. In contrast to G. pulex, G. tigrinus sustains prolonged K+ stress by using its higher capacity of aerobic metabolism to stabilize energy metabolism. [References: 9]