Until recently, only a few microsatellites have been available for Cucurbita, thus their development is highly desirable. The Austrian oil-pumpkin variety Gleis-dorfer Olkiirbis (C. pepo subsp. pepo) and the C. moschata cultivar Soler (Puerto Rico) were used for SSR development. SSR-enriched partial genomic libraries were established and 2,400 clones were sequenced. Of these 1,058 (44%) contained an SSR at least four repeats long. Primers were designed for 532 SSRs; 500 primer pairs produced fragments of expected size. Of these, 405 (81%) amplified polymorphic fragments in a set of 12 genotypes: three C. moschata, one C. ecuadorensis, and eight C. pepo representing all eight cultivar groups. On an average, C. pepo and C. moschata produced 3.3 alleles per primer pair, showing high inter-species transferability. There were 187 SSR markers detecting polymorphism between the USA oil-pumpkin variety "Lady Godiva" (O5) and the Italian crookneck variety "Bianco Friulano" (CN), which are the parents of ourprevious F_2 mapping population. It has been used to construct the first published C. pepo map, containing mainly RAPD and AFLP markers. Now the updated map comprises 178 SSRs, 244 AFLPs, 230 RAPDs, five SCARs, and two morphological traits (h and B). It contains 20 linkage groups with a map density of 2.9 cM. The observed genome coverage (Co) is 86.8%.
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机译：直到最近，葫芦科只有少数微卫星可用，因此非常需要发展它们。奥地利的油南瓜品种Gleis-dorfer Olkiirbis（C. pepo subsp。pepo）和C. moschata品种Soler（Puerto Rico）用于SSR开发。建立了富含SSR的部分基因组文库，并对2,400个克隆进行了测序。在这些1,058个（44％）包含SSR中，至少有四个重复序列。设计用于532个SSR的引物; 500对引物产生了预期大小的片段。其中，有405个（81％）扩增了12种基因型的多态片段：三个代表所有八个品种组的Mo. C. moschata，一个C. ecuadorensis和八个C. pepo。平均而言，C。pepo和C. moschata每个引物对产生3.3个等位基因，显示出高的种间转移性。在美国油南瓜品种“ Lady Godiva”（O5）和意大利圆领品种“ Bianco Friulano”（CN）之间有187个SSR标记检测多态性，它们是我们以前的F_2作图种群的父母。它已被用来构建第一个公开的梭状芽孢杆菌图谱，主要包含RAPD和AFLP标记。现在，更新后的图谱包含178个SSR，244个AFLP，230个RAPD，5个SCAR和2个形态特征（h和B）。它包含20个连锁组，图密度为2.9 cM。观察到的基因组覆盖率（Co）为86.8％。
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