Genetic map for pumpkin Cucurbita pepo using random amplified polymorphic DNA markers

摘要

A genetic map for Cucurbita pepo (2n=40) would be a useful tool for immediate application in plant breeding and comparative genetic analysis. Using Random Amplified Polymorphic DNA (RAPDs), Simple Sequence Repeats (SSRs) and phenotypic/morphological traits, a molecular map for pumpkin was constructed. The map was developed using an F_2 population obtained from a cross between an Austrian oil-pumpkin inbred line (SZG1) and a zucchini genotype resistant to ZYMV. From a total of 280 loci, 254 RAPD markers, 24 of which are co-dominant, 3 SSRs and one qualitative trait could be mapped at a LOD score of 3 and maximum distance of 35; for the time being, 22 markers are unlinked. The map covers 1425 cM and contains 36 linkage groups with 17 of them includingless than 4 markers. The average distance between markers is 5.5 cM. However, a number of gaps (>20 cM) are still to be filled. One-way ANOVA analysis revealed a significant association of RAPD markers with fruit length that accounts for more than 40% of the variation of this trait.