Sequence analysis of the novel allele HLA-B*070502.

摘要

We here describe the identification of the novel allele HLA-B*070502, which has been detected in a potential bone marrow donor. The HLA class I typing results were A*24,*29; B*070502,*5701. Initial testing at the HLA-B locus was performed by an external institute by two independent techniques -PCR-SSO (sequence-specific oligonu-cleotides) and PCR-SSP (sequence-specific primers). No match result was obtained by PCR-SSO due to the detection of one inconclusive reaction, whereas the unambiguous result HLA-B*0713 was obtained by PCR-SSP. The inconclusive reaction pattern in PCR-SSO and the detection of a rare allele in PCR-SSP suggested the presence of a new allele, which was further investigated by PCR-SBT. Cycle sequencing of exons 2 and 3 was performed following haplotype-specific amplification essentially as previously described (1) using an Applied Biosystems (Foster City, CA, USA) 3730 sequencer, resulting in the detection of the new allele.