Cadmium pathology in an insect cell line: ultrastructural and biochemical effects.

摘要

Cadmium (Cd) pathology was studied in an insect cell line (Aedes albopictus C6/36) at the ultrastructural level. The most prominent pathological changes occurred at the level of the nucleus: chromatin clumping, indentations, filling and dilatation of the perinuclear cisternae and an increased amount of bound ribosomes were observed. In the cytoplasm, condensation and swelling of mitochondria, increase of both free and membrane-bound ribosomes, filling and dilatation of the rough endoplasmic reticulum, and increase of the lysosomal system were the most conspicuous effects. The increased content of the perinuclear and cytoplasmic cisternae was probably due to an increased protein synthesis or a disturbance of the protein export system. This picturediffered clearly from the osmotically swollen electron-lucent cisternae that have been described in other pathological situations. The enhancement of the lysosomal system was paralleled by a slight but significant stimulation of the acid phosphatase activity in the sublethal Cd concentration range. In vitro experiments suggested that Cd probably acts directly on this enzyme. Abnormal medium acidification in cultures treated with low Cd levels was correlated with an increased production of lactic acid. Together with the morphological data, a Cd-induced impairment of the aerobic metabolism is suggested.