Immunohistochemical localization of ecdysteroid receptor and ultraspiracle in the epithelial cell line from Chironomus tentans (Insecta, Diptera).

摘要

Ecdysteroid receptor (EcR) and its heterodimerization partner, ultraspiracle (USP), were demonstrated in an epithelial cell line from Chironomus tentans [Camptochironomus tentans] by immunohistochemistry. In untreated cells both proteins were present in nuclei as well as in granular compartments of the cytosol. At 1 day after addition of 1-鍹 20-OH-ecdysone (20E) total immunofluorescence had increased in the nuclei, whereas the cytoplasmic staining had disappeared. At the 2nd and 3rd days all cellswithin a vesicle appeared identical according to morphological criteria, but the EcR and USP immunoreactivity became restricted to patches of neighbouring cells. The hormonally induced changes in the pattern of localization of functional ecdysteroid [moulting hormone] receptors, the heterodimer of EcR and USP, are discussed in relation to similar effects of 20E on acetylcholinesterase and muscarinic acetylcholine receptor distribution in this cell line.