In vitro effects of ascorbic acid and beta-glycerophosphate on human gingival fibroblast cells

摘要

Ascorbic acid (AA) and beta-glycerophosphate (0G) are considered in vitro osteogenic factors important to the differentiation of osteoblastic progenitor and dental pulp cells into mineralized tissue-forming cells. So, the present study investigated invitro if these mineralizing inducible factors (AA and beta G) could influence differentiation of human gingival fibroblasts when compared with human pulp cells and osteogenic cells derived from rat calvaria cultured. The expression of osteopontin (OPN)and osteoadherin (OSAD) was analyzed by indirect immunofluorescence, immunocytochemistry as well as Western-blotting. In addition, the main ultrastructural aspects were also investigated. No mineralized matrix formation occurred on gingival fibroblasts induced with AA+beta G. On these cells, no expression of OPN and OSAD was observed when compared with pulp cells, pulp cells induced with AA+beta G as well as osteogenic cells. Ultrastructure analysis additionally showed that gingival fibroblasts exhibited typical fibroblast morphology with no nodule formation. The present findings showed that AA and beta G could not promote a mineralized cell differentiation of human gingival fibroblasts and confirm that human dental pulp cells, as the osteogenic cells,are capable to form a mineralized extracellular.