The effect on preimplantation embryo development of non-specific inflammation localized outside the reproductive tract

摘要

The aim of this study was to evaluate the possible effect of non-specific acute inflammation localized outside the reproductive tract on the quality of preimplantation embryos In fertilized female mice two experimental models of inflammation were used -trinitrobenzene sulfonic acid colitis and carrageenan paw oedema Inflammation was induced during the cleavage period of embryo development and embryos were collected at 92 h post hormonal synchronization Stereomicroscopical evaluation of in vivo derived embryos showed that the presence of inflammation in the maternal body did not affect their basic developmental abilities, 1 e there were no significant differences in the proportion of early blastocysts, morulas, slowly developing embryos and degenerates between embryonic pools obtained from mothers with induced inflammation and control mothers In the next step, non-degenerated embryos from all mothers were cultured in vitro under standard conditions for another 24 h, and the average cell number (fluorescence DNA staining) and the incidence of cell death (fluorescence viability staining combined with TUNEL assay) were evaluated The majority of cultured embryos reached expanded blastocyst stage There were no significant differences in the average cell numbers of blastocysts but blastocysts derived from mothers with induced inflammation showed a significantly higher incidence of dead cells in both experiments The majority of dead cells were of apoptotic origin These results show that non-specific inflammation localized outside the reproductive tract has no detrimental effect on the preimplantation embryo growth, however It can affect the embryo quality