Effectiveness of slow freezing and vitrification for long-term preservation of mouse ovarian tissue

摘要

This study was conducted to evaluate the interaction between eryo-damage and ART outcome after cryopreservation of mouse ovarian tissues with different methods. Either a vitrification or a slow freezing was employed for the cryopreservation of B6CBAF1 mouse ovaries and follicle growth and the preimplantation development of intrafollieular oocytes following parthenogenesis or IVF were monitored. Both eryopreservation protocols caused significant damage to follicle components, including vacuole formation and mitochondrial deformities. Regardless of the ciyopreservation protocols employed, a sharp (P < 0.0001) decrease in follicle viability and post-thaw growth was detected. When INF program was employed, significant (P < 0.05) decrease in cleavage and blastocyst formation was notable in both modes of cryopreservation. However, such retardation was not found when oocytes were parthenogenetically activated. In the IVF oocytes, slow freezing led to better development than vitrification. In conclusion, a close relationship between cryopreservation and ART methods should be considered for the selection of eryopreservation program.