Sperm factors related to in vitro penetration of porcine oocytes

摘要

This study was designed to evaluate the relationship between sperm factors and penetration capacity in an in vitro system with immature porcine oocytes. The sperm parameters evaluated in 145 ejaculates were volume, sperm concentration, total cells in the ejaculate, ATP content, morpho-anomalies, percentage of motile sperm cells, forward progressive motitity (FPM), acrosome status (NAR), hypo-osmotic swelling test (HOS), osmotic resistance test (ORT), eosin-nigrosin viability stain and sperm membrane integrity (DCF). Porcine oocytes (a total of 8736) were used to evaluate the capacity of the different sperm assays to predict penetration. Many parameters were found to be related to in vitro penetration ability; all conventional semen parameters, except sperm concentration and eosin-nigrosin staining, were significantly better in high (>75%) than in low penetration rates (<75%). When the ejaculates were preselected the number of significantly related parameters was lower.