Production of Glutathione and Reactive Oxygen Species in Cell Line HT-1080 Treated with Chub Mackerel (Scomber japonicus) Extract

摘要

To evaluate the antioxidative activity of mackerel extracts, we examined the effect of mackerel extract on the production of glutathione (GSH) and reactive oxygen species (ROS) in HT-1080 cells. GSH levels were measured by using a fluorescent method employing monobromobiman (mBBr), and cellular oxidative stress caused by ROS generation from H2O2 was measured by performing the dichlorofluorescein-diacetate (DCFH-DA) assay. Treatments with acetone+methylene chloride (A+M) and methanol (MeOH) extracts both at 1 and 5 mg mL(-1) concentrations resulted in a significant increase in GSH levels compared with the control. The intracellular GSH levels also increased significantly in the presence of n-hexane and 85% aqueous MeOH fractions. The treatments with A+M and MeOH extracts (0.5 mg mL(-1) concentrations) inhibited H2O2-induced ROS production compared with the control without the extracts by 7.4% and 10.5%, respectively. Of all the fractions, the effect of 85% aqueous MeOH fraction was the strongest in reducing the concentration of ROS. Our results demonstrated that the 85% aqueous MeOH fraction inhibited cellular oxidation and that this fraction may contain valuable active compounds.