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首页> 外文期刊>The Veterinary Journal >Evaluation of rapid assays for the detection of bovine coronavirus, rotavirus A and Cryptosporidium parvum in faecal samples of calves
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Evaluation of rapid assays for the detection of bovine coronavirus, rotavirus A and Cryptosporidium parvum in faecal samples of calves

机译:快速检测牛犊粪便样本中牛冠状病毒,轮状病毒A和小隐孢子虫的检测方法的评估

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摘要

Rapid immunochromatographic assays for detecting infections with bovine coronavirus (BCV), rotavirus A and Cryptosporidium parvum in calf faeces were evaluated using as gold standards a reverse transcriptase polymerase chain reaction (BCV and rotavirus) and a sedimentation-flotation technique (C. parvum). Rapid tests for the detection of BCV and rotavirus showed a high specificity (96.4% and 95.3%, respectively), but a relatively low sensitivity (60.0% and 71.9%, respectively). Sensitivity and specificity for detection of C. parvum were high (100% and 94.6%, respectively).
机译:使用逆转录酶聚合酶链反应(BCV和轮状病毒)和沉降浮选技术(C. parvum)作为金标准,评估了用于检测牛粪中牛冠状病毒(BCV),轮状病毒A和小隐孢子虫感染的快速免疫色谱法。快速检测BCV和轮状病毒的方法显示出高特异性(分别为96.4%和95.3%),但灵敏度相对较低(分别为60.0%和71.9%)。检测小隐孢子虫的敏感性和特异性很高(分别为100%和94.6%)。

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