首页> 外文期刊>The Journal of Physiology >Long- and short-term intravital imaging reveals differential spatiotemporal recruitment and function of myelomonocytic cells after spinal cord injury
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Long- and short-term intravital imaging reveals differential spatiotemporal recruitment and function of myelomonocytic cells after spinal cord injury

机译:长期和短期的活体成像显示脊髓损伤后骨髓单核细胞的差异性时空募集和功能

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摘要

After spinal cord injury (SCI), resident and peripheral myelomonocytic cells are recruited to the injury site and play a role in injury progression. These cells are important for clearing cellular debris, and can modulate the retraction and growth of axons in vitro. However, their precise spatiotemporal recruitment dynamics is unknown, and their respective roles after SCI remain heavily debated. Using chronic, quantitative intravital two-photon microscopy of adult mice with SCI, here we show that infiltrating lysozyme M (LysM(+)) and resident CD11c(+) myelomonocytic cells have distinct spatiotemporal recruitment profiles, and exhibit changes in morphology, motility, phagocytic activity and axon interaction patterns over time. This study provides the first in vivo description of the influx of inflammatory and resident myelomonocytic cells into the injured spinal cord and their interactions with cut axons, and underscores the importance of precise timing and targeting of specific cell populations in developing therapies for SCI.
机译:脊髓损伤(SCI)后,常驻和外周骨髓单核细胞被募集到损伤部位,并在损伤进展中起作用。这些细胞对于清除细胞碎片很重要,并且可以在体外调节轴突的收缩和生长。然而,它们确切的时空募集动力学尚不清楚,并且在SCI之后它们各自的作用仍存在很多争议。使用SCI成年小鼠的慢性定量活体双光子显微镜检查,我们发现浸润的溶菌酶M(LysM(+))和常驻CD11c(+)骨髓单核细胞具有独特的时空募集特征,并且在形态,运动性,随着时间的推移吞噬活性和轴突相互作用模式。这项研究提供了炎性和驻留的骨髓单核细胞流入受损脊髓及其与切下的轴突的相互作用的第一个体内描述,并强调了精确时机和针对特定细胞群的靶向在开发SCI治疗中的重要性。

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