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Functional connectivity of the main intercalated nucleus of the mouse amygdala.

机译:小鼠杏仁核的主要插入核的功能连接。

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Intercalated cells (ITCs) of the amygdala are clusters of GABAergic cells that surround the basolateral complex of the amygdala (BLA). Growing evidence suggests that ITCs are required for the expression of fear extinction. The main intercalated nucleus (Im) is the largest of the ITC clusters and could also be important for emotional processing. We used whole-cell recordings from Im neurons in acute slices of mouse amygdala. We found that these neurons were medium-sized spiny projection cells. Their passive and active membrane responses were consistent with those previously reported in other ITC clusters. The axon of Im neurons was, in many cases, cut at the slice boundaries, suggesting long-range projections. Axonal branches could be detected in several amygdala nuclei where they made functional synapses. We also functionally studied Im cell inputs. Excitatory postsynaptic currents (eEPSCs) were evoked by the stimulation of the Im, intermediate capsula (IC), external capsula (EC) or BLA, when GABAergic transmission was pharmacologically blocked. An occlusion test indicated that fibres recruited by stimulating Im and IC, or Im and EC were distinct. These eEPSCs had both NMDA and AMPA receptor components. Inhibitory postsynaptic currents (eIPSCs) were evoked after the stimulation of the Im, the EC and the BLA, when glutamatergic transmission was pharmacologically blocked. Furthermore, dopamine reversibly hyperpolarised, and decreased the firing frequency and the input resistance of Im cells via dopamine type 1 receptor. Our data suggest that the Im is functionally connected to other amygdala nuclei and is under neuromodulatory influence. We propose that the Im serves as key neuronal substrate of fear extinction.
机译:杏仁核的插层细胞(ITC)是围绕杏仁核(BLA)基底外侧复合体的GABA能细胞簇。越来越多的证据表明,表达恐惧消灭需要ITC。主插入核(Im)是ITC簇中最大的,对于情绪处理也可能很重要。我们在小鼠杏仁核的急性切片中使用了来自Im神经元的全细胞记录。我们发现这些神经元是中等大小的棘突细胞。它们的被动和主动膜反应与先前在其他ITC群集中报道的反应一致。在许多情况下,Im神经元的轴突在切片边界处被切开,表明存在远距离投射。轴突分支可以在几个杏仁核中检测到,在那里它们产生功能性突触。我们还在功能上研究了Im细胞的输入。当药理学上阻止GABA能传递时,Im,中间包膜(IC),外部包膜(EC)或BLA的刺激引起兴奋性突触后电流(eEPSC)。阻塞试验表明,通过刺激Im和IC或Im和EC募集的纤维是不同的。这些eEPSC具有NMDA和AMPA受体成分。 Im,EC和BLA的刺激在药理学上阻断了谷氨酸能传递后,引起了抑制性突触后电流(eIPSC)。此外,多巴胺可逆超极化,并通过1型多巴胺受体降低了Im细胞的放电频率和输入电阻。我们的数据表明,Im在功能上与其他杏仁核相关,并且处于神经调节作用下。我们建议Im作为恐惧绝种的关键神经元底物。

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