In Vitro Comparison of Light-Emittieg Diodes and Carnoslc Acid Effects on Keratinocyte Proliferation and Wound Healing

摘要

Introduction: Light-emitting diode (LED) therapy uses different wavelengths of light and has been reported to accelerate cutaneous wound healing. Carnosic acid is an antioxidant that is also thought to be photoprotective. We designed an in vitro study to examine the effects of LED and carnosic acid on the proliferation and migration of human keratinocytes. Materials and Methods: Clinically normal human keratinocytes were cultured arid exposed to two wavelengths: 620 nm and 660 nm LED at different fluences. In the second part of this study, a different batch of human keratinocytes was grown in culture, and different concentrations of carnosic acid were added. Results: At the two wavelengths that were used, LED did not appear to have any therapeutic effect and was not effective in stimulating keratinocyte proliferation. Exposure to greater energy levels (increased fluence) produced increased cell damage that was directly proportional to the increase in energy. On the other hand, treatment of the cell cultures with the antioxidant carnosic acid resulted in an increase of keratinocyte cell proliferation, and this increase was also proportional to the concentration of carnosic acid. Conclusions: This study did not support the hypothesis that LED treatment results in keratinocyte proliferation; however, carnosic acid, a potent antioxidant, stimulated keratinocyte production and could be implicated in wound healing and rejuvenation.