Cytotoxicity of cultured macrophages exposedto antimicrobial zinc oxide (ZnO) coatingson nanoporous aluminum oxide membranes

摘要

Zinc oxide (ZnO) is a widely used commercial material that is finding use in wound healing applications due to itsantimicrobial properties. Our study demonstrates a novel approach for coating ZnO with precise thickness control onto20 nm and 100 nm pore diameter anodized aluminum oxide using atomic layer deposition (ALD). ZnO was depositedthroughout the nanoporous structure of the anodized aluminum oxide membranes. An 8 nm-thick coating of ZnO,previously noted to have antimicrobial properties, was cytotoxic to cultured macrophages. After 48 h, ZnO-coated 20nm and 100 nm pore anodized aluminum oxide significantly decreased cell viability by ≈65% and 54%, respectively,compared with cells grown on uncoated anodized aluminum oxide membranes and cells grown on tissue culture plates.Pore diameter (20–200 nm) did not influence cell viability.