The importance of gene-centring microarray data.

摘要

In the April, 2010, issue of The Lancet Oncology, Weigelt and colleagues1 investigate the association between three different methods of predicting molecular subtypes of breast cancer, all referred to as single sample predictors (SSPs). Their conclusions, however, are flawed by the use of uncentred microarray data. The original methods are all based on their correlation to expression centroids generated from three different gene lists. Weigelt and colleagues1 apply the methods to four different gene-expression datasets without performing the essential gene centring procedures before classification.