Factors affecting the production efficiency of transgenic rats.

摘要

Transgenic rats were produced by pronuclear microinjection with 42 DNA constructs. The efficiency of production of transgenic rats ranged from 0.73 to 3.53% of the microinjected eggs. Promoter region, structural gene, chain length, and strand ends of the exogenous DNA did not affect production efficiency. The optimum concentration of exogenous DNA was 5 鎔/ml. The donor strain for the pronuclear eggs was a possible factor in production efficiency.