OFF-LINE AND ON-LINE SOLID-PHASE EXTRACTION FOLLOWED BY LIQUID CHROMATOGRAPHY FOR THE DETERMINATION OF PRIORITY PHENOLS IN NATURAL WATERS

摘要

Two analytical methods have been developed for the determination in water of 18 priority phenolics listed in US EPA method 604 and on EEC list 76/464. A solid-phase extraction system using eight different sorbents packed in a precolumn was coupled on-line with a liquid chromatograph with UV detection. The ensuing method uses 50-100 mL of ground water; its performance was compared with that of an off-line method using Empore extraction disks and 1 L water samples. Phenol recoveries varied from < 20 to 100% for concentrations in the range 0.1-10 mu g/L at an acid pH. The presence of the phenols in water was confirmed by using thermospray LC-mass spectrometry in the negative ion mode. The stability of the phenols in water was studied at a 10 mu g/l level in ground and estuarine water at acid pH (2.5-3) and at 4 degrees C for 1 month. The system was validated by various interlaboratory exercises with samples containing 2,4,6-trichlorophenol and pentachlorophenol at concentrations from 0.1 to 0.5 mu g/L. [References: 27]