Nicotinic Receptors Differentially Regulate N-Methyl-D-aspartate Damage in Acute Hippocampal Slices

摘要

Although in neuronal cultures nicotine was reported to prevent early and delayed excitotoxic death, no studies with nicotinic drugs have been done with acute hippocampal slices. We investigated the effect of nicotine and methyllycaconitine (MLA) on the toxicity of N-methyl-D-aspartate (NMDA) in the CA1 area of hippocampal slices. The excitotoxic effect of NMDA was assessed as decreased recovery of the capability to produce synaptically evoked population spikes (PSs). Application of nicotine or MLA before NMDA application increased the recovery of PSs. This electrophysiological recovery was used as a measure of ht early neuroprotective events. The neuroprotection conferred by both nicotine and MLA was inhibited by dihydro-beta-erythroidine, showing mediation of neuroprotection by alpha4beta2 neuronal nicotinic receptors (nAChRs). Because nicotine activates alpha4beta2 and other nAChRs subtypes, whereas 10 nM MLA inhibits the alpha7 subtype, we propose the involvement of a neuronal circuitry-dependent mechanism for nicotinic neuroprotection. The effect of nicotine downstream from the receptors was investigated using inhibitors of cell signaling. The results suggest that the effect of nicotine is mediated by tyrosine receptor kinases, 1,2-phosphatidylinositol-3 kinase, and the mitogen-activated extracellular signal-regulated kinases. Although nicotine neuroprotection is Ca~(2+)-dependent, neither L-type Ca~(2+) channels nor calmodulin-dependent protein kinase is involved in the effect of nicotine. In summary, these results suggest that in acute slices nicotine. In summary, these results suggest that in acute slices nicotinic protection is initiated either by direct activation of alpha4beta2 or indirectly by inhibition of alpha7 followed by signal transudation involving tyrosine kinases, phospholipid-dependent kinases, and mitogen-activated kinases.