Developers for TLC of Triterpene Glycosides

摘要

Color reactions are used to detect triterpene glycosides on TLC plates because pure triterpene glycosides are colorless compounds. The reactions involve aromatic aldehydes in strong mineral acids [1-3], a mixture of acetic anhydride and conc. H_2SO_4 (Liebermann-Burchard reagent) [1, 3, 4], Ce(SO_4)_2 or Fe(III) salts in inorganic acids [1], SbCl_3 [1, 5], and phosphomolybdic acid [1, 4]. Triterpene glycosides are also detected in chromatograms using H_2SO_4 or phosphotungstic acids of various concentrations, a mixture of chlorosulfonic acid acetic acids, or hemolysis of erythrocytes [1, 5].