An improved method for testing weak VOCs in dry plants with a purge and trap concentrator coupled to GC-MS

摘要

The purge and trap (P&T) technique was improved for measuring the release of organic compounds with weak volatility (weak VOCs) from dry plant materials. Using distilled water as a dispersant, the plant tissues were mulled and placed in the purge tube of a P&T concentrator. Then the sample-containing purge tube was heated to 80 C with helium as the carrier gas, and the purged volatiles were preconcentrated in the trap prior to analysis with GC-MS. The VOCs in Chinese herbal medicinal plants Swertia tetraptera, Saussurea involucrate and S. lacostei, which had been stored dry for 1-2.5 years were assayed with this improved method and conventional P&T techniques. Our results show this new P&T method had great promise for determining the VOCs in dry plant materials. Using this new technique, we identified 38 weak VOCs with a large peak area from the dry samples. In contrast, less than five VOCs were detected by the conventional P&T method. So the improved heat-purge and trap system showed to be more efficient for measuring the release of the weak VOCs from dry plant materials.