Determination of trace Sudan IV residues in foods through molecularly imprinted SPE coupled with LC

摘要

In this paper, a highly selective Sudan IV molecularly imprinted polymer was synthesized by surface molecular imprinting technique in combination with a sol-gel process using γ-aminopropyl triethoxysilane as functional monomer, tetraethoxysilane as cross-linker and activated silica gel as support material. The imprinted polymer was characterized by FT-IR spectra, scanning electron micrograph and adsorption experiments and it was exhibited good recognition and selective ability, offered a faster rate for the adsorption of Sudan IV. Using the imprinted material as sorbent, a solid-phase extraction coupled with high-performance liquid chromatography method for determination of trace Sudan IV was presented. The detection limit (S/N = 3) was 25.2 ng L~(-1), and the RSD for five replicate was 2.86%. With a loading flow rate of 2.5 mL min~(-1) for loading 30 mL, an enrichment factor of 104 was achieved. This method was applied for extraction and determination of chilli powder and duck egg samples with good recoveries ranging from 85.3 to 98.1%.