Effects of NaCl Stress on Callus Induction and Plant Regeneration from Mature Seeds of Rice (Oryza sativa L.)

摘要

Two commercial indica rice varieties, PAU 201 and PR 116, exhibited appreciable callus induction in vitro but the capacity to induce and regenerate callus decreased under salinity stress in both when exposed to sodium chloride (NaCl) at 90 mM. Calluswas induced on semisolid MS medium supplemented with 2.5 mg/1 2,4-dichlorophenoxyacetic acid + 0.5 mg/1 kinetin + 560 mg/1 proline + 30 g/1 sucrose + 8 g/1 agar. Callus developed shoots on MS medium supplemented with 2.0 mg/1 benzylamino purine + 0.5 mg/1 kinetin + 0.5 mg/1 a-naphthalene acetic acid + 30 g/1 sucrose + 8 g/1 agar. The results indicated the need to optimize the protocol for callus induction, maintenance and regeneration before selection process for tolerance to salinity.