Interlaboratory measurement differences in intracellular Carbovir triphosphate concentrations in HIV-infected patients: sources of variability in processing, shipping, and quantitation.

摘要

Dual nucleoside reverse-transcriptase inhibitors (NRTIs) form the backbone of most potent antiretroviral (ARV) regimens. The quantity of dideoxynucleotide analog triphosphates (ddNTPs) produced appears to be tightly regulated by a number of host cellular factors. Differences in nucleoside drug metabolism may explain, in part, the significant interindividual variability observed in intracellular ddNTP concentrations with several NRTIs, including zidovudine (ZDV), lamivudine (3TC) tenofovir disoproxil fumarate (TDF), and abacavir (ABC). However, the issue of measurement error due to variability during specimen processing and ddNTP quantitation should be considered when interpreting clinical nucleotide concentrations obtained in different laboratories despite similar analytical methodologies.