STUDIES ON THE BIOSYNTHESIS OF BIALAPHOS - BIOCHEMICAL MECHANISM OF C-P BOND FORMATION - DISCOVERY OF PHOSPHONOPYRUVATE DECARBOXYLASE WHICH CATALYZES THE FORMATION OF PHOSPHONOACETALDEHYDE FROM PHOSPHONOPYRUVATE

摘要

The biosynthetic step following the phosphoenolpyruvate (PEP) phosphomutase reaction which forms a C-P bond of bialaphos was proven by the identification of phosphonopyruvate (PnPy) and phosphonoacetaldehyde (PnAA) as intermediates in the culture broth of Streptomyces hygroscopicus, a producing organism of bialaphos, and by detection of enzymatic decarboxylation of PnPy to PnAA. Purified PnPy decarboxylase turned out to require thiamine diphosphate and Mg2+ as cofactors. PnPy decarboxylase drives the unfavorable forward reaction to form PnPy catalyzed by PEP phosphomutase and is suggested to be essential to C-P compound biosynthesis. [References: 30]