Effect of Cyclen on the Prolongation of M Intermediate Lifetime in the D96N Mutant of Bacteriorhodopsin

摘要

A new kind of chemical additive-cyclen (1,4,7,10-tetra-azacyclododecane) was used in BR_(D96N)-PVA films in an attempt to obtain longer M state lifetime. At the highet cyclen concentration of 1:250, the lifetime of M state could be prolonged to 936s. Comparing the lifetime of the M state in BR_(D96N) suspension and BR_(D96N)-PVA film with the same cyclen content, it is the strong complexing properties of cyclen towards H~+ that plays the most important role in this prolongation.