Genotypic variability for weight, storage loss and germination of microtubers in potato (Solanum tuberosum L.).

摘要

Healthy tubers of seven genotypes of potato (MF-I, MF-II, TPS-7, TPS-13, TPS-67, JTH/C-107 and EX/A-680-16) were obtained and planted in pots in the greenhouse. After 3-4 weeks, the shoot tip and axillary node explants were inoculated. The plantlets were multiplied on solid multiplication medium through shoot tip and axillary node culture, and elongated in liquid multiplication medium. After 3-4 weeks, the multiplication medium was replaced by microtuber induction medium supplemented with four BAP levels (6, 8, 10, and 12 mg/litre) and two sucrose rates (75 and 85 g/litre). The genotypes differed significantly with mean weight from 0.41 to 1.00 g. Genotype MF-II was significant over MF-I, TPS-7, TPS-67, JTH/C-107 and EX/A-680-16. The genotype TPS-7 was significant over MF-I, TPS-67, JTH/C-107 and EX/A-680-16. Minimum and maximum storage loss was observed in MF-II and TPS-67, respectively. Maximum and minimum germination was recorded in genotypes MF-II and TPS-67, respectively. It is concluded that genotype, sucrose and BAP had pronounced effects on weight, storage loss and germination of microtubers..