Effect of different capacitating agents on in vitro fertilization of goat follicular oocytes

摘要

The present investigation is an attempt to establish optimal culture conditions for in vitro fertilization (IVF) of goat oocytes Ovaries were obtained from a slaughter house and cumulus oocytes complexes (COCs) were washed three times in TL-HEPES medium and were matured for 24 h in TCM-199 (M-199) media supplemented with 10% fetal calf serum (FCS) and hormones (1 mg/ml 17β estradiol, E_2; 0.5 mg/ml ml follicle stimulating hormones (FSH) and 50 mg/ml leutinizing hormone, LH) After 24 hrs. of incubation oocytes were inseminated with percoll separated epididymal spermatozoa in Brackett and Oliphant (BO) or Talp-fertilization medium and treated with either Ca~(++) ionophore (A-23187) (0.1, 0.2 and 0.5 mM/ml + 5mM caffeine / L ) or heparin (0,1,10 and 100 mg/ml) + 5mM caffeine / L or heparin (0,1,10 and 100mg/ml) alone in Talp-fertilization medium.