Superovulatory responses of eCG and live births following surgical embryo transfer in Marwari ewes

摘要

Multiple ovulation and embryo collection (n = 8) was done in Marwari ewes followed by theit transfer to synchronised recipients (n = 7) Donor ewes were superovulated by injecting 1000 IU of eCG on day 12 of a progesterone implant under two groups Group 1 (n = 4) received a commercially available progestagen implant CIDR-G whereas in Group II (n = 4) ewes received an indigenously prepared implant Ewes were induced to estrus by a PG injection and mated to fertile rams at estrus Embryos were collected surgically on day 6, their ovarian response recorded and embryos transferred surgically. Only 75% (3/4) ewes responded in both the groups. The number of CL/ewe, total ova recovery rate, fertilized eggs/ewe and number of morula/blastocysts/ewe were 7.0±251, 4.66±2.66, 3.66±2 18 and 3.0±1.52 in group I and 10.0±5 13, 9.34±4.37, 7.34+4.48 and 9.34+2.02 in Group II Statistically all these were non significantly different except for the total ova recovery rate which was significantly higher (P < 0.05)in Group II indicating thereby that locally made sponges can replace commercial implants of progestagens. Three pregnancies and live births resulted from transfer of 12 embryos to 7 recipients.