Activation of Double-stranded DNA by One pcPNA Strand for Its Site-selective Scission with Ce~(IV)/EDTA

摘要

In the presence of only one strand of pseudo-complementary PNA (pcPNA), double-stranded DNA was selectively hydrolyzed by Ce~(IV)/EDTA (EDTA = ethylenediamine-N,N,N',N'-tetraacetate) at target site. Compared with previously reported artificial restriction DNA cutters using two pcPNA strands, the present cutters are simple and promising for various applications.