EPA inhibits the inhibitor of kappaB alpha (I kappaB alpha)/NF- kappaB/muscle RING finger 1 pathway in C2C12 myotubes in a PPAR gamma-dependent manner.

摘要

The present study was conducted to evaluate the mechanism by which n-3 PUFA regulates the inhibitor of kappaB alpha (I kappaB alpha)/NF- kappaB/muscle RING finger 1 (MuRF1) pathway in C2C12 myotubes. After treatment with 150, 300 or 600 muM- alpha-linolenic acid (ALA) or -EPA for 24 h in C2C12 myotubes, the levels of phosphorylated I kappaB alpha (p-I kappaB alpha) and total I kappaB alpha were measured by Western blot. Compared with the bovine serum albumin (BSA) control, 150 and 300 muM-ALA and -EPA, respectively, did not affect the total I kappaB alpha protein level (P