KINETIC MODELLING OF CELL GROWTH, SUBSTRATE UTILIZATION, AND BIOSURFACTANT PRODUCTION FROM SOLID AGROWASTE (BETA VULGARIS) BY BACILLUS LICHENIFORMIS STK 01

摘要

The kinetics of cell growth, substrate utilization, and biosurfactant production by Bacillus licheniformis STK 01 from a solid agrowaste substrate (Beta vulgaris) and a refined substrate (mineral salts, MS) was investigated. Data obtained were fitted to the integrated Monod equation, logistic models, and Leudeking-Piret model using nonlinear regression analyses. The maximum cell growth was observed after 72 h of fermentation for both substrates. The highest biosurfactant production was 5.8 +/- 0.5 g/L when the B. vulgaris waste substrate was used, while the production increased up to 9.78 +/- 1.02 g/L when MS was used. The biosurfactant produced from B. vulgaris and MS lowered the surface tension of the broth to 30 and 23.5 mN/m respectively. Furthermore, from the kinetic data analyses, cell growth and B. vulgaris utilization were described by the logistic model and modified Monod equation, respectively, with the maximum cell growth rate of 0.026 h(-1), cell yield of 0.617, and the Monod saturation constant being 0.418 g/L. Similarly, biosurfactant production was best described by the logistic model while the production rate constant was 0.140 h(-1). This study is applicable, among other areas, in the design of biological systems augmented with B. vulgaris waste.