Proteins that bind specific DNA sequences play critical roles in gene expression and genome modification. The ability to design such proteins to function at any desired sequence would unlock numerous applications in research, biotechnology and medicine. Recent advances in the field of engineered zinc finger proteins (ZFPs) have made it possible to realise this potential. Using well-validated design methods, ZFPs can be assembled that specifically bind to virtually any DNA sequence. This capability allows targeting of proteins and peptides to any genome location via fusion with an appropriate ZFP. This review will briefly summarise the structural properties, engineering methods and applications of designed ZFPs.
展开▼
