Amaranth Seed Oil: Effect of Oral Administrationon Energetic Functions of Rat Liver Mitochondria Activated with Adrenaline

摘要

Respiration parameters of liver mitochondria (MCh) in rats fed with amaranth seed oil for 3 weekshave been evaluated. Thirty minutes before decapitation, adrenaline was injected intraperitoneally at a low dose(350 gg/kg body weight) to both control and experimental animals. It was shown that in animals that wereinjected with adrenaline and did not receive oil, the rate of phosphorylating respiration increased by 32% andphosphorylation time decreased by 22% upon oxidation of succinate; upon oxidation of α-ketoglutarate in thepresence of the succinate dehydrogenase inhibitor malonate, phosphorylating respiration was activated by 23%.The respiration of MCh upon oxidation of succinate + glutamate and α-ketoglutarate in the absence of malonatewas not affected by adrenaline. The intake of oil markedly activated almost all parameters of mitochondrial res-piration in experimental rats upon oxidation of all above-listed substrates in both coupled and uncoupled MCh.However, phosphorylation time was close to the control value (upon oxidation of succinate) or increased (uponoxidation of α-ketoglutarate in the presence and absence of malonate). The injection of adrenaline to animalsreceiving oil did not affect the oil-activated respiration of MCh oxidizing the substrates used; however, phos-phorylation time in all groups of animals decreased. Ca~(2+)capacity of MCh in rats receiving amaranth oil didnot change. Thus, our data show that feeding of rats with amaranth oil activates mitochondrial respiration andprevents MCh hyperactivation induced by adrenaline.