'Switch-off' biosensing for chymotrypsin-catalyzed reaction by SPR-SERS spectroscopy

Fu C.; Xu W.; Chen G.; Xu S.

首页> 外文期刊>The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry >'Switch-off' biosensing for chymotrypsin-catalyzed reaction by SPR-SERS spectroscopy

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'Switch-off' biosensing for chymotrypsin-catalyzed reaction by SPR-SERS spectroscopy

机译：SPR-SERS光谱法对胰凝乳蛋白酶催化反应的“关闭”生物传感

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We describe a novel 'switch-off' biosensing strategy for the detection of chymotrypsin based on surface plasmon resonance (SPR) and surface-enhanced Raman scattering (SERS) spectroscopy. This approach analyzes the fingerprint spectrum of the chymotrypsin-catalyzed substrate. The lowest probed concentration is 0.4 nM for chymotrypsin.

机译：我们描述了一种基于表面等离振子共振（SPR）和表面增强拉曼散射（SERS）光谱检测胰凝乳蛋白酶的新型“关闭”生物传感策略。该方法分析了胰凝乳蛋白酶催化的底物的指纹图谱。胰凝乳蛋白酶的最低探测浓度为0.4 nM。

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《The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry》 |2013年第21期|共5页
作者
Fu C.; Xu W.; Chen G.; Xu S.;
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正文语种 eng
中图分类分析化学;
关键词
'Switch-off'; SPR-SERS spectroscopy; detection;

机译：“关闭”;SPR-SERS光谱;检测;

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1. 'Switch-off' biosensing for chymotrypsin-catalyzed reaction by SPR-SERS spectroscopy [J] . Fu C., Xu W., Chen G., The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry . 2013,第21期

机译：SPR-SERS光谱法对胰凝乳蛋白酶催化反应的“关闭”生物传感
2. Raman spectroscopy in the study of chymotrypsin-catalyzed reactions [J] . Balakhnina Irina A., Brandt Nikolay N., Chikishev Andrey Yu, Vibrational Spectroscopy: An International Journal devoted to Applications of Infrared and Raman Spectroscopy . 2020,第期

机译：胰凝乳蛋白酶催化反应研究中的拉曼光谱
3. Impedance spectroscopy and conductometric biosensing for probing catalase reaction with cyanide as ligand and inhibitor [J] . Bouyahia N., Hamlaoui M.L., Hnaien M., Bioelectrochemistry . 2011,第2期

机译：阻抗光谱和电导生物传感，用于探测以氰化物为配体和抑制剂的过氧化氢酶反应
4. P1.20: Novel QCM Biosensing Based on Analyte-Specific Precipitation Reactions [C] . IEEE IEEE International Conference on Sensors . 2002

机译：P1.20：基于分析物特异性降水反应的新型QCM生物溶解
5. Molecular Understanding of Biointerfacial Behavior for Biosensing and High-Temperature Interfacial Chemical Reactions in Pyrolysis [D] . Samieegohar, Mohammadreza. 2017

机译：热解中生物传感和高温界面化学反应的生物界面行为的分子理解
6. The optical detection of transients in trypsin- and chymotrypsin-catalyzed reactions. [O] . S A Bernhard, H Gutfreund 1965

机译：胰蛋白酶和胰凝乳蛋白酶催化反应中瞬态的光学检测。
7. The optical detection of transients in trypsin- and chymotrypsin-catalyzed reactions. [O] . Bernhard, S A, Gutfreund, H 1965

机译：胰蛋白酶和胰凝乳蛋白酶催化反应中瞬态的光学检测。
8. Application of Ion Cyclotron Resonance Spectroscopy, Photoionization Mass Spectrometry and Photoelectron Spectroscopy to Study the Properties and Reactions of Ions in Gases [R] . Beauchamp, J. L. 1981

机译：离子回旋共振光谱，光电离质谱和光电子能谱在气体中离子性质和反应中的应用

'Switch-off' biosensing for chymotrypsin-catalyzed reaction by SPR-SERS spectroscopy

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