HPLC Quantitative Analysis Method for the Determination of alpha-Mangostin in Mangosteen Fruit Rind Extract

摘要

The fruit rind extract of mangosteen {Garcinia mangostana Linn.) of which a major active component is alpha-mangostin, has been popularly used in food supplements and herbal cosmetic preparations. alpha-Mangostin is used as a marker quantitative analysis and standardization of the raw materials and preparation from this plant. The precise method for analysis of plant constituents is normally a reverse-phase high performance liquid chromatographic (RP-HPLC). The aims of this study were to develop andvalidate a RP-HPLC method for determination of a-mangostin content in the extracts of mangosteen fruit rind. Chromatographic separation was carried out on a Hypersil~R BDS C-18 column (4.6 x 250 mm, 5 urn) at room temperature using a gradient mobile phase consisting of 70-80 % acetonitrile in 0.1 %v/v ortho phosphoric acid at the flow rate of 1 mL min~(-1) with a UV detection at 320 nm. The method was validated for linearity, precision, accuracy, limit of detection (LOD), and limit of quantitation (LOQ). The linearity of the proposed method was found in the range of 10-200 ug mL~(-1) with regression coefficient 0.9999. Intraday and interday precision studies showed the relative standard deviation less than 2 %. Accuracy of the method was determined bya recovery study conducted at 3 different levels, and the average recovery was 100.01 %. The LOD and LOQ were 0.06 and 0.17 mu g mL~(-1), respectively. Two samples of mangosteen fruit rind were separately extracted and analyzed using validated HPLC method. The contents of a-mangostin in the crude extracts and dried powder were within the ranges of 8.36 - 10.04 and 1.84 - 2.47 %w/w, respectively. This developed HPLC method was proven to be precise, specific, sensitive, and accurate for routine quality assessment of raw material of mangosteen fruit rind, its extract, and products.