Association of transforming growth factor-beta1 gene polymorphisms with genetic susceptibility to acute myocardial infarction.

摘要

INTRODUCTION: Transforming growth factor beta 1 (TGF-beta1) gene plays an important role in acute myocardial infarction (AMI); however, little is known about the relation of variations within the gene and risk of cardiovascular diseases. In this study, the authors evaluated the influence of TGF-beta1 polymorphisms on the onset and progression of AMI in Iranian patients comparing with healthy individuals. METHODS: Genomic DNA and peripheral blood mononuclear cells of 900 enrolled patients with AMI and 900 control subjects were extracted. The -509 C/T, 868T/C, 913G/C and 11929C/T TGF-beta1 polymorphisms were detected. The messenger RNA (mRNA) expression and serum levels of TGF-beta1 were analyzed by real-time reverse-transcriptase polymerase chain reaction and ELISA, respectively. RESULTS: The frequency of "T" allele in -509 C/T, "C" allele in 868T/C, "C" allele in 913G/C and "T" allele in 11929C/T polymorphisms were significantly higher in the patients than control subjects (P < 0.001). There were significant differences in circulating levels of TGF-beta1 in the patients than in control subjects (P < 0.001). These concentrations are associated with its gene polymorphism. The mRNA expression levels of TGF-beta1 were significantly higher in the patient serums compared with controls (P < 0.001). CONCLUSIONS: Our results confirmed the association between the TGF-beta1 polymorphisms and risk of AMI, which suggest that genetic polymorphisms in TGF-beta1 might be helpful for determining susceptibility to AMI in Iranian patients. There are also significant relationship between serum TGF-beta1 and occurrence of AMI. In addition, susceptibility to AMI might be related to TGF-beta1 gene expression, which affects its serum levels.