Enzymatic resolution of (RS)-1-phenylalkyl β-D-glucosides to (R)-1-phenylalkyl β-primeverosides and (S)-1-phenylalkyl β-D-glucosides via plant xylosyltransferase

摘要

The stereoselective xylosylation of (RS)-1-phenylalkyl β-D-glucosides was investigated using plant xylosyltransferase isolated from cultured Catharanthus roseus cells. Enzymatic xylosylation of (RS)-1-phenylethyl β-D-glucoside afforded (R)-1-phenylethyl β-primeveroside and (S)-1-phenylethyl β-D-glucoside. The (R)-selective xylosylation of (RS)-1-phenylbutyl β-D-glucoside also occurred to give (R)-1-phenylbutyl β-primeveroside and recovered (S)-1-phenylbutyl β-D-glucoside.