Evaluation of luminol-H2O2-KIO4 chemiluminescence system and its application to hydrogen peroxide, glucose and ascorbic acid assays.

摘要

Hydrogen peroxide can be directly determined by a luminol-KIO4-H2O2 CL system. The detection limit was 3.0 X 10-8 mol/litre and the calibration graph was linear over the range of 2.0X10-7-6.0X10-4 mol/litre. The relative standard deviation of H2O2 was 1.1% for 2.0X10-6 mol/litre (n=11). Glucose was indirectly determined through measuring the H2O2 generated by the oxidation of glucose in the presence of glucose oxidase at pH 7.6. The present method provides a source for H2O2, which, in turn, coupledwith the luminol-KIO4-H2O2 CL reaction system. The CL was linearly correlated with glucose concentration of 0.6-110μg/ml. The relative standard deviation was 2.1% for 10μg/ml (n=11). Detection limit of glucose was 0.08μg/ml. Ascorbic acid was also indirectly determined by the suppression of luminol-KIO4-H2O2 CL system. The calibration curve was linear over the range of 1.0X10-7-1.0X10-5 mol/litre of ascorbic acid. The relative standard deviation was 1.0% for 8.0X10-7 mol/litre (n=11). Detection limit of ascorbic acid was 6.0X10-8 mol/litre. These proposed methods have been applied to determine glucose and ascorbic acid in tablets and injection.