PNA Encoding (PNA = peptide nucleic acid):From Solution-Based Libraries to Organized Microarrays

摘要

Microarray-based technologies have attracted attention in chemical biology by virtue of their miniaturized format,which is well suited to probe ligand-pro-tein interactions or investigate enzymatic activity in complex biological mixtures.A number of research groups have reported the preparation of surfaces on mi-croarrays with specific functional groups to chemoselec-tively attach small molecules from libraries.We have developed an alternative method whereby libraries are encoded with peptide nucleic acid (PNA),such that libraries which exist as mixtures in solution self-assemble into an organized microarray through hybridization to produce readily available DNA arrays.This allows libraries synthesized by split and mix methods to be decoded in a single step.An asset of this method compared to direct spotting is that libraries can be used in solution for bioassays prior to self-assembly into the microarray format.