Why development of new HPLC column technology is still alive Stationary phase innovations rise to meet the demands of modern chromatographers

摘要

Tne challenges facing today's analytical chemists are discussed in the context of modern advancements in HPLC columns. The chromatographic technology described in this article includes sub 2 urn phases, fused core particles, silica hydride materials, microano LC separation systems, and mixed mode phases. Advantages and disadvantages of each technique are contrasted. No one stationary phase material can address all of the analytical problems of today; rather, each technology has its own strengths suited to particular applications and method goals. Among these goals are sensitivity requirements in fields such as metabolomics and biotechnology, enhanced selectivity/resolution, low cost, and high throughout in clinical or pharmaceutical assays.