DEVELOPMENT OF ANALYTICAL METHODS FOR HPLC-UV TO THE ANTIMICROBIANS TETRACYCLINE, SULFAMETHOXAZOLE AND TRIMETHOPRIM USING ZIRCONOCENE-BASED SILICA AS PRE-CONCENTRATION/EXTRACTION SYSTEMS

摘要

DEVELOPMENT OF ANALYTICAL METHODS FOR HPLC-UV TO THE ANTIMICROBIANS TETRACYCLINE, SULFAMETHOXAZOLE AND TRIMETHOPRIM USING ZIRCONOCENE-BASED SILICA AS PRE-CONCENTRATION/EXTRACTION SYSTEMS. This paper evaluates the adsorption capacity of zirconocene-based silica materials in the pre-concentration of antimicrobians (tetracycline, sulfamethoxazole and trimethoprim) in aqueous medium. These materials were prepared by grafting the zirconocene onto silicas pre-treated at different temperatures. The retention capacity of these materials was evaluated by off line SPE and HPLC-UV and the proposed methodology was validated in ultrapure, tap and river water. The recovery for tetracycline was 72% (in the solid phase A) and, for sulfamethoxazole and trimethoprim was 68 and 95% in the commercial C18, respectively. The target antimicrobians were not detected in the Arroio Diluvio (Porto Alegre - RS).