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首页> 外文期刊>Pulmonary pharmacology & therapeutics >Influence of hyperbaric oxygen on tumor necrosis factor-alpha and nitric oxide production in endotoxin-induced acute lung injury in rats.
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Influence of hyperbaric oxygen on tumor necrosis factor-alpha and nitric oxide production in endotoxin-induced acute lung injury in rats.

机译:高压氧对内毒素诱导的大鼠急性肺损伤中肿瘤坏死因子-α和一氧化氮产生的影响。

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摘要

We previously demonstrated that hyperbaric oxygen (HBO) treatment alleviated lipopolysaccharide (LPS)-induced acute lung injury in rats. However, the mechanisms responsible for the protective effect are still not fully understood. To obtain further information on the protective effect of HBO, in this study we investigated the role of tumor necrosis factor-alpha (TNF-alpha) and nitric oxide (NO) in intratracheal spraying LPS-induced acute lung injury in rats after HBO or hyperoxia treatment. The results showed that HBO but not hyperoxia attenuated the TNF-alpha level in plasma and bronchoalveolar lavage (BAL) fluid, NO concentration in BAL and plasma, and inducible NO synthase protein expression in lung tissue based on the Western blotting and immunohistochemical staining.
机译:我们先前证明高压氧(HBO)处理可减轻脂多糖(LPS)诱导的大鼠急性肺损伤。但是,引起保护作用的机制仍不完全清楚。为了获得有关HBO保护作用的进一步信息,在这项研究中,我们研究了肿瘤坏死因子-α(TNF-alpha)和一氧化氮(NO)在气管内喷洒LPS诱导的HBO或高氧大鼠急性肺损伤中的作用。治疗。结果表明,基于Western印迹和免疫组化染色,HBO可以减轻血浆和支气管肺泡灌洗液(BAL)中的TNF-α水平,BAL和血浆中的NO浓度,并诱导肺组织中可诱导的NO合酶蛋白表达,而不会降低高氧血症。

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