Involvement of pregnane xenobiotic receptor in mating-induced allopregnanolone formation in the midbrain and hippocampus and brain-derived neurotrophic factor in the hippocampus among female rats

摘要

Rationale Given that the pregnane neurosteroid, 5alpha-pregnan-3alpha-ol-20-one (3alpha,5alpha-THP), is increased following behavioral challenges (e.g., mating), and that there is behavioral-induced biosynthesis of 3alpha,5alpha-THP in midbrain and mesocorticolimbic structures, 3alpha,5alpha-THP likely has a role in homeostasis and motivated reproduction and reproduction-related behaviors (e.g., affect, affiliation). The role of pregnane xenobiotic receptor (PXR), involved in cholesterol metabolism, for these effects is of continued interest.Objectives We hypothesized that there would be differences in brain levels of 3alpha,5alpha-THP following varied behavioral experiences, an effect abrogated by knockdown of PXR in the midbrain.Methods Proestrous rats were infused with PXR antisense oligonucleotides (AS-ODNs) or vehicle to the ventral tegmen-tal area before different behavioral manipulations and assessments. Endpoints were expression levels of PXR in the midbrain, 3alpha,5alpha-THP, and ovarian steroids (estradiol, progesterone, dihydroprogesterone) in the midbrain, striatum, hippocampus, hypothalamus, prefrontal cortex, and plasma. Results Across experiments, knocking down PXR reduced PXR expression and 3alpha,5alpha-THP levels in the midbrain and hippocampus. There were differences in terms of the behavioral manipulations, such that paced mating had the most robust effects to increase 3alpha,5alpha-THP levels and reduce open field exploration and social interaction. An additional question that was addressed is whether brain-derived neurotrophic factor (BDNF) is a downstream factor for regulating effects of behavioral-induced 3alpha,5alpha-THP biosynthesis. Rats infused with PXR AS-ODNs had lower levels of BDNF in the hippocampus. Conclusion Thus, PXR may be a regulator of mating-induced 3alpha,5alpha-THP formation and behavioral changes and neural plasticity, such as BDNF.