Optimization of the Conditions for Preparation of Silyl Derivatives of Phenylcarboxylic Acids in Diethyl and Methyl tert-Butyl Ethers

摘要

In this work, the optimum conditions of derivatization of six phenylcarboxylic acids (sepsis biomarkers) in diethyl ether and methyl tert-butyl ether were selected. N,O-Bis(trimethylsilyl)trifluoroacetamide (BSTFA) was used as a silylating agent. The following conditions were chosen as optimum in terms of sensitivity of the silylating agent and its amount: 300 mu L of ether and 80 mu L of BSTFA, at which the derivatization yield of the studied compounds in model solutions was more than 70% in diethyl ether and more than 85% in methyl tert-butyl ether. The developed conditions will make it possible to increase the derivatization yield at various sample preparation procedures (liquid-liquid or solid-phase extraction) compared to the currently used conditions.