Morphometric and molecular analyses of the composition of potato cyst nematodes carried out by the State Plant Health and Seed Inspection Service during the years 2009-2013

摘要

Laboratories of the State Plant Health and Seed Inspection Service to assess the occurrence of the Potato Cyst Nematodes {Globodera rostochiensis and G. pallida) over the period 2009-2013, analysed 213,161 soil samples taken from fields on Polish territory. Viable cysts (ie. containing living eggs and juveniles) from Globodera genus were extracted from 951 soil samples (0.45%). The results of identification of cysts detected in 584 samples (61.41%) were verified using molecular methods. These cysts were preliminary identified to species G. rostochiensis - 547 samples, G. artemisiae - 35 samples and a mixture of two species G. rostochiensis and G. pallida in 1 sample, based on morphometric characters. The result of identification of cysts in one sample was vague. Molecular tests confirmed the results of identification based on morphometric characters. Similarly one of the tested cyst samples where identification results were unclear was finally identified by molecular tests to G. pallida. In addition, cysts detected in the soil attached to two samples of table potatoes originating from Cyprus, were identified to G. rostochiensis and G. pallida species by morphometric characters and with molecular tests.