Detection of potato viruses directly in tuber extracts - comparison of ELISA and immuno-captured RT-PCR.

Treder K.; Pilecki T.; ycus P.

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Detection of potato viruses directly in tuber extracts - comparison of ELISA and immuno-captured RT-PCR.

机译：直接在块茎提取物中检测马铃薯病毒-ELISA和免疫捕获RT-PCR的比较。

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The application of the cocktail-ELISA procedure allowed for the detection of PLRV, PVM and PVY in samples 10-fold more diluted than by DAS-ELISA. Dilutions of extracts obtained from PLRV-infected tuber were also tested by IC-RT-PCR and PLRV-specific PCR product was observed even for the most diluted samples.

机译：鸡尾酒-ELISA程序的应用使得检测到的稀释液中的PLRV，PVM和PVY比DAS-ELISA稀释了10倍。还通过IC-RT-PCR测试了从感染PLRV的块茎中提取的提取物的稀释液，即使对于稀释度最高的样品，也观察到了PLRV特异性PCR产物。

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《Progress in Plant Protection》 |2009年第2期|共6页
作者
Treder K.; Pilecki T.; ycus P.;
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正文语种 pol
中图分类农业科学;
关键词
detection; diagnostic techniques; ELISA; plant pathogens; plant viruses; potatoes; tubers; Carlavirus; Flexiviridae; Luteoviridae; Polerovirus; Potato leafroll virus; Potato virus M; Potato virus Y; Potyviridae; Potyvirus; Solanum tuberosum;

机译：检测;诊断技术;ELISA;植物病原体;植物病毒;马铃薯;块茎;Carlavirus;Flexiviridae;utetoviridae;杆状病毒;马铃薯卷叶病毒;马铃薯M;

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1. Detection of potato viruses directly in tuber extracts - comparison of ELISA and immuno-captured RT-PCR. [J] . Treder K., Pilecki T., ycus P. Progress in Plant Protection . 2009,第2期

机译：直接在块茎提取物中检测马铃薯病毒-ELISA和免疫捕获RT-PCR的比较。
2. Detection of potato mop-top virus in soils and potato tubers using bait-plant bioassay, ELISA and RT-PCR. [J] . Muhammad Arif, Murad Ali, Anayatur Rehman, Journal of Virological Methods . 2014,第Null期

机译：使用诱饵植物生物测定，ELISA和RT-PCR检测土壤和马铃薯块茎中的马铃薯拖把病毒。
3. Simultaneous detection of potato viruses, PLRV, PVA, PVX and PVY from dormant potato tubers by TaqMan real-time RT-PCR. [J] . Agindotan BO, Shiel PJ, Berger PH Journal of Virological Methods . 2007,第1a2期

机译：通过TaqMan实时RT-PCR同时检测休眠马铃薯块茎中的马铃薯病毒，PLRV，PVA，PVX和PVY。
4. THE COMPARISON OF IEF AND ELISA FOR THE DETECTION OF INSECTICIDE RESISTANCE IN FIELD POPULATIONS OF THE PEACH-POTATO APHID MYZUS PERSICAE IN NORTHERN ENGLAND [C] . Erica J. Little International Congress of Pesticide Chemistry . 1986

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5. Tuber Necrosis-causing Viruses of Potato: Genetic Diversity and Host-pathogen Interactions [D] . Moyo, Lindani 2018

机译：马铃薯块茎坏死病毒：遗传多样性和宿主-病原体相互作用
6. Characterization of Monoclonal Antibodies Specific for Erwinia carotovora subsp. atroseptica and Comparison of Serological Methods for Its Sensitive Detection on Potato Tubers [O] . María Teresa Gorris, Benito Alarcon, María M. Lopez, 1994

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7. Detection of Dickeya spp. latent infection in potato seed tubers using PCR or ELISA and correlation with disease incidence in commercial field crops under hot-climate conditions [O] . L. Tsror Lahkim, O. Erlich, M. Hazanovsky, 2011

机译：检测Dickeya SPP。使用PCR或ELISA的马铃薯种子块茎潜伏感染以及在热气候条件下与商业领域作物中的疾病发病率相关

Detection of potato viruses directly in tuber extracts - comparison of ELISA and immuno-captured RT-PCR.

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